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recombinant βngf  (R&D Systems)


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    R&D Systems recombinant βngf
    Recombinant βngf, supplied by R&D Systems, used in various techniques. Bioz Stars score: 92/100, based on 5 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/ngf+%CE%B2+cf+rat/Recombinant+Rat+beta-NGF+Protein%2C+CF/pmc11827847__jci___135___183873___s034-12-0-2
    Average 92 stars, based on 5 article reviews
    recombinant βngf - by Bioz Stars, 2026-10
    92/100 stars

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    Recombinant:

    Article Title: THE TrkA RECEPTOR MEDIATES EXPERIMENTAL THERMAL HYPERALGESIA PRODUCED BY NERVE GROWTH FACTOR : modulation by the p75 neurotrophin receptor
    Article Snippet: .. NGF-β (rat, recombinant) (Cat No.N-2513, Sigma-Aldrich, St. Louis MO, USA), or NGF-β/CF (rat) (R&D Systems, Inc., MN, USA) was dissolved in phosphate buffered saline (PBS; In Vitrogen) as a stock solution (1000 ng/10 μL) and stored in small aliquots at −80°C. ..

    Article Title: THE TrkA RECEPTOR MEDIATES EXPERIMENTAL THERMAL HYPERALGESIA PRODUCED BY NERVE GROWTH FACTOR : modulation by the p75 neurotrophin receptor
    Article Snippet: .. Drugs NGF-β (rat, recombinant) (Cat No.N-2513, Sigma-Aldrich, St. Louis MO, USA), or NGF-β/CF (rat) (R&D Systems, Inc., MN, USA) was dissolved in phosphate buffered saline (PBS; In Vitrogen) as a stock solution (1000 ng/10 μL) and stored in small aliquots at −80°C. ..

    Saline:

    Article Title: THE TrkA RECEPTOR MEDIATES EXPERIMENTAL THERMAL HYPERALGESIA PRODUCED BY NERVE GROWTH FACTOR : modulation by the p75 neurotrophin receptor
    Article Snippet: .. NGF-β (rat, recombinant) (Cat No.N-2513, Sigma-Aldrich, St. Louis MO, USA), or NGF-β/CF (rat) (R&D Systems, Inc., MN, USA) was dissolved in phosphate buffered saline (PBS; In Vitrogen) as a stock solution (1000 ng/10 μL) and stored in small aliquots at −80°C. ..

    Article Title: THE TrkA RECEPTOR MEDIATES EXPERIMENTAL THERMAL HYPERALGESIA PRODUCED BY NERVE GROWTH FACTOR : modulation by the p75 neurotrophin receptor
    Article Snippet: .. Drugs NGF-β (rat, recombinant) (Cat No.N-2513, Sigma-Aldrich, St. Louis MO, USA), or NGF-β/CF (rat) (R&D Systems, Inc., MN, USA) was dissolved in phosphate buffered saline (PBS; In Vitrogen) as a stock solution (1000 ng/10 μL) and stored in small aliquots at −80°C. ..



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    FIGURE 2 | XPS survey scans of (A) PCL scaffold; (B) PCL air treated scaffold (PCL Air scaffold); (C) PCL + NH2 + scaffold; (D) PCL+ NH2 + + Heparin scaffold. Carbon was detected at 285 eV, oxygen at 532 eV, nitrogen at 400 eV, and sulphur at 168 eV.

    Journal: Frontiers in Materials

    Article Title: Bioactive 3D Scaffolds for the Delivery of NGF and BDNF to Improve Nerve Regeneration

    doi: 10.3389/fmats.2021.734683

    Figure Lengend Snippet: FIGURE 2 | XPS survey scans of (A) PCL scaffold; (B) PCL air treated scaffold (PCL Air scaffold); (C) PCL + NH2 + scaffold; (D) PCL+ NH2 + + Heparin scaffold. Carbon was detected at 285 eV, oxygen at 532 eV, nitrogen at 400 eV, and sulphur at 168 eV.

    Article Snippet: 2.4 Immobilisation of Nerve Growth Factor, Brain Derived Neurotrophic Factor and Nerve Growth Factor Plus Brain Derived Neurotrophic Factor to Polycaprolactone + NH2 + + Heparin Scaffolds NGF (Cat. No. 556-NG/CF, R&D systems, United States), BDNF (Cat. No. 248-BD/CF, R&D systems, United States) and a combination of both (NGF plus BDNF) were immobilised onto PCL + NH2 + + Heparin scaffolds.

    Techniques:

    FIGURE 3 | NGF and BDNF release from NH2 + + Heparin. (A) NH2 + + Heparin + Immobilised NGF 1 ng/ml (circles), and NH2 + (squares) at different time points, from 1 to 504 h (21 days). NGF at 1 ng/ml in solution in PBS was used as a control (triangles). (B) BDNF release from NH2 + + Heparin + Immobilised BDNF 1 ng/ml (circles), and NH2 + (squares) at different time points, from 1 to 504 h (21 days). BDNF at 1 ng/ml in solution was used as a control (triangles).

    Journal: Frontiers in Materials

    Article Title: Bioactive 3D Scaffolds for the Delivery of NGF and BDNF to Improve Nerve Regeneration

    doi: 10.3389/fmats.2021.734683

    Figure Lengend Snippet: FIGURE 3 | NGF and BDNF release from NH2 + + Heparin. (A) NH2 + + Heparin + Immobilised NGF 1 ng/ml (circles), and NH2 + (squares) at different time points, from 1 to 504 h (21 days). NGF at 1 ng/ml in solution in PBS was used as a control (triangles). (B) BDNF release from NH2 + + Heparin + Immobilised BDNF 1 ng/ml (circles), and NH2 + (squares) at different time points, from 1 to 504 h (21 days). BDNF at 1 ng/ml in solution was used as a control (triangles).

    Article Snippet: 2.4 Immobilisation of Nerve Growth Factor, Brain Derived Neurotrophic Factor and Nerve Growth Factor Plus Brain Derived Neurotrophic Factor to Polycaprolactone + NH2 + + Heparin Scaffolds NGF (Cat. No. 556-NG/CF, R&D systems, United States), BDNF (Cat. No. 248-BD/CF, R&D systems, United States) and a combination of both (NGF plus BDNF) were immobilised onto PCL + NH2 + + Heparin scaffolds.

    Techniques: Control

    FIGURE 4 | Average neurite length and Schwann cell migration of DRG when cultured on PCL scaffolds, PCL Air scaffolds, PCL + NH2 + scaffolds, PCL + NH2 + + Heparin scaffolds, and PCL + NH2 + + Heparin + Immobilised neurotrophin for 7 days. (A) Average neurite length and (B) Schwann cell migration for when DRG were cultured with NGF. NGF was immobilised on the bioactive surfaces at 1 pg/ml, 1 ng/ml, 10 ng/ml, 100 ng/ml, and 1 µg/ml. NGF was added in solution at 1 µg/ml with control surfaces. (C) Average neurite length and (D) Schwann cell migration for when DRG were cultured with BDNF. BDNF was immobilised on the bioactive surfaces at 1 pg/ml, 1 ng/ml, 10 ng/ml, 100 ng/ml, and 1 µg/ml. BDNF was added in solution at 1 µg/ml with control surfaces. (E) Average neurite length and (F) Schwann cell migration for when DRG were cultured with NGF plus BDNF. NGF plus BDNF were immobilised on the bioactive surfaces at 1 and 100 ng/ml. NGF plus BDNF were added in solution at 1 µg/ml with control surfaces. One-way ANOVA statistical analysis was performed with Tukey procedure of multiple comparisons *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001.

    Journal: Frontiers in Materials

    Article Title: Bioactive 3D Scaffolds for the Delivery of NGF and BDNF to Improve Nerve Regeneration

    doi: 10.3389/fmats.2021.734683

    Figure Lengend Snippet: FIGURE 4 | Average neurite length and Schwann cell migration of DRG when cultured on PCL scaffolds, PCL Air scaffolds, PCL + NH2 + scaffolds, PCL + NH2 + + Heparin scaffolds, and PCL + NH2 + + Heparin + Immobilised neurotrophin for 7 days. (A) Average neurite length and (B) Schwann cell migration for when DRG were cultured with NGF. NGF was immobilised on the bioactive surfaces at 1 pg/ml, 1 ng/ml, 10 ng/ml, 100 ng/ml, and 1 µg/ml. NGF was added in solution at 1 µg/ml with control surfaces. (C) Average neurite length and (D) Schwann cell migration for when DRG were cultured with BDNF. BDNF was immobilised on the bioactive surfaces at 1 pg/ml, 1 ng/ml, 10 ng/ml, 100 ng/ml, and 1 µg/ml. BDNF was added in solution at 1 µg/ml with control surfaces. (E) Average neurite length and (F) Schwann cell migration for when DRG were cultured with NGF plus BDNF. NGF plus BDNF were immobilised on the bioactive surfaces at 1 and 100 ng/ml. NGF plus BDNF were added in solution at 1 µg/ml with control surfaces. One-way ANOVA statistical analysis was performed with Tukey procedure of multiple comparisons *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001.

    Article Snippet: 2.4 Immobilisation of Nerve Growth Factor, Brain Derived Neurotrophic Factor and Nerve Growth Factor Plus Brain Derived Neurotrophic Factor to Polycaprolactone + NH2 + + Heparin Scaffolds NGF (Cat. No. 556-NG/CF, R&D systems, United States), BDNF (Cat. No. 248-BD/CF, R&D systems, United States) and a combination of both (NGF plus BDNF) were immobilised onto PCL + NH2 + + Heparin scaffolds.

    Techniques: Migration, Cell Culture, Control

    FIGURE 5 | DRG seeded on control PCL bioactive scaffolds: PCL scaffold, PCL air treated scaffold (PCL Air scaffold), PCL + NH2 + scaffold, PCL + NH2 + + Heparin scaffold, PCL + NH2 + + Heparin + Immobilised NGF at 1 ng/ml scaffold, PCL + NH2 + + Heparin + Immobilised BDNF at 1 ng/ml scaffold, and PCL air treated scaffold (PCL Air scaffold) with NGF plus BDNF at 1 µg/ml in culture medium. B-III tubulin protein in neurites stained in green. S100β of Schwann cells stained in red. Merged channels are yellow, representing the co-localisation of growing neurites and Schwann cells. Scale bar 500 µm.

    Journal: Frontiers in Materials

    Article Title: Bioactive 3D Scaffolds for the Delivery of NGF and BDNF to Improve Nerve Regeneration

    doi: 10.3389/fmats.2021.734683

    Figure Lengend Snippet: FIGURE 5 | DRG seeded on control PCL bioactive scaffolds: PCL scaffold, PCL air treated scaffold (PCL Air scaffold), PCL + NH2 + scaffold, PCL + NH2 + + Heparin scaffold, PCL + NH2 + + Heparin + Immobilised NGF at 1 ng/ml scaffold, PCL + NH2 + + Heparin + Immobilised BDNF at 1 ng/ml scaffold, and PCL air treated scaffold (PCL Air scaffold) with NGF plus BDNF at 1 µg/ml in culture medium. B-III tubulin protein in neurites stained in green. S100β of Schwann cells stained in red. Merged channels are yellow, representing the co-localisation of growing neurites and Schwann cells. Scale bar 500 µm.

    Article Snippet: 2.4 Immobilisation of Nerve Growth Factor, Brain Derived Neurotrophic Factor and Nerve Growth Factor Plus Brain Derived Neurotrophic Factor to Polycaprolactone + NH2 + + Heparin Scaffolds NGF (Cat. No. 556-NG/CF, R&D systems, United States), BDNF (Cat. No. 248-BD/CF, R&D systems, United States) and a combination of both (NGF plus BDNF) were immobilised onto PCL + NH2 + + Heparin scaffolds.

    Techniques: Control, Staining